Validity of the Enzyme-linked Immunoelectrotransfer Blot (EITB) for naturally acquired porcine cysticercosis

CM Jayashi; AE Gonzalez; R Castillo Neyra; S Rodríguez; HH García; MW Lightowlers

Journal article

Validity of the Enzyme-linked Immunoelectrotransfer Blot (EITB) for naturally acquired porcine cysticercosis

CM Jayashi, AE Gonzalez, R Castillo Neyra, S Rodríguez, HH García, MW Lightowlers

Veterinary Parasitology | Published : 2014

DOI: 10.1016/j.vetpar.2013.10.004

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Abstract

The Enzyme-linked Immunoelectrotransfer Blot (EITB) has been used widely as a screening test for Taenia solium cysticercosis in swine. However, the relation between seropositivity and infection in pig populations from endemic areas has not been well defined. The aim of this study is to relate EITB seropositivity with infection and infection burden, analyse the trade-off between sensitivity and specificity with various cut-off points for the EITB assay, and finally describe the serology changes in a cohort of rural pigs raised under natural conditions. A group of 107 pigs that were used as controls during a vaccination field trial in Peru was our study population. The prevalence of porcine cy..

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University of Melbourne Researchers

Marshall Lightowlers Author

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Awarded by National Health and Medical Research Council

Funding Acknowledgements

The authors thank the Cysticercosis Working Group in Peru staff in Tumbes, and the staff of the Veterinary Preventive Medicine Laboratory at the Veterinary Faculty of San Marcos University in Lima, for their technical support and assistance. The authors thank especially Gianfranco Arroyo, Eduardo Barron, Juan Huaman and Juan Chanta for their outstanding support with the field work. Drs Patty Wilkins and Victor Tsang generously supplied the cysticercus antigen preparation that was used in the EITB assays. Funding from the Australian National Health and Medical Research Council (grant numbers 350279, 628320, 100354) is acknowledged. Hector H. Garcia is a Wellcome Trust Senior International Research Fellow.